Dockerfile

FROM ubuntu:16.04
LABEL author="onur.yukselen@umassmed.edu"  description="Docker image containing all requirements for the dolphinnext/rnaseq pipeline"

ENV LANG=C.UTF-8 LC_ALL=C.UTF-8
ENV PATH /opt/conda/bin:$PATH


RUN apt-get update --fix-missing && \
    apt-get install -y wget bzip2 ca-certificates curl git libtbb-dev g++
    
RUN wget --quiet https://repo.anaconda.com/miniconda/Miniconda3-4.5.11-Linux-x86_64.sh -O ~/miniconda.sh && \
    /bin/bash ~/miniconda.sh -b -p /opt/conda && \
    rm ~/miniconda.sh && \
    /opt/conda/bin/conda clean -tipsy && \
    ln -s /opt/conda/etc/profile.d/conda.sh /etc/profile.d/conda.sh && \
    echo ". /opt/conda/etc/profile.d/conda.sh" >> ~/.bashrc && \
    echo "conda activate base" >> ~/.bashrc
    
# configure image 
RUN apt-get -y update 
RUN apt-get -y install software-properties-common build-essential
RUN add-apt-repository 'deb https://cloud.r-project.org/bin/linux/ubuntu xenial-cran40/'
RUN apt-key adv --keyserver hkp://keyserver.ubuntu.com:80 --recv-keys E298A3A825C0D65DFD57CBB651716619E084DAB9
RUN apt-get -y install apt-transport-https
RUN apt-get -y update

RUN apt-get update && apt-get install -y gcc unzip 
RUN curl "https://awscli.amazonaws.com/awscli-exe-linux-x86_64-2.0.30.zip" -o "awscliv2.zip"
RUN unzip awscliv2.zip
RUN ./aws/install
RUN aws --version

RUN apt-get clean && \
    rm -rf /var/lib/apt/lists/*

COPY environment.yml /
RUN conda update -n base -c defaults conda
RUN conda env create -f /environment.yml && conda clean -a
# Install dolphin-tools
RUN git clone https://github.com/dolphinnext/tools /usr/local/bin/dolphin-tools
RUN mkdir -p /project /nl /mnt /share
ENV PATH /opt/conda/envs/dolphinnext-rnaseq-3.0/bin:/usr/local/bin/dolphin-tools/:$PATH

RUN pip install "multiqc==1.7"
RUN cd /bin && wget https://github.com/ChristopherWilks/megadepth/releases/download/1.1.0/megadepth && chmod 777 megadepth
RUN conda create -n umi_tools_env -c bioconda umi_tools==1.0.1