##Please let me know if you have any problems. ##The TSMR analysis are separated into IV selection(GCTA_COJO_analysis.sh) and MR analysis(TSMR.R). ##The selected IVs were presented in the Table S2 for gut microbiome and Table S3 for stroke and its subtypes in our study.(When utilized the IVs, please cite our paper). ##In our code, we taked the information in EAS as example.