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<!DOCTYPE html>
<html>
<head>
<meta charset="utf-8">
<title>Strobe-enhanced microscopy stage</title>
<meta http-equiv="X-UA-Compatible" content="IE=edge">
<meta name="viewport" content="width=device-width, initial-scale=1">
<link rel="shortcut icon" href="./assets/favicon.ico" />
<link href="./static/style.css" rel="stylesheet">
<link href="./assets/style.css" rel="stylesheet">
</head>
<body>
<header class="site-header">
<div class="wrapper header-wrapper" >
<div class=header-text>
<h1 class="site-title">Strobe-enhanced microscopy stage</h1>
<p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p>
</div>
</div>
</header>
<div class="page-content">
<div>
<nav class="sidebar">
<a href="#" class="menu-icon">
<svg viewBox="0 0 18 15">
<path fill="#424242" d="M18,1.484c0,0.82-0.665,1.484-1.484,1.484H1.484C0.665,2.969,0,2.304,0,1.484l0,0C0,0.665,0.665,0,1.484,0 h15.031C17.335,0,18,0.665,18,1.484L18,1.484z"/>
<path fill="#424242" d="M18,7.516C18,8.335,17.335,9,16.516,9H1.484C0.665,9,0,8.335,0,7.516l0,0c0-0.82,0.665-1.484,1.484-1.484 h15.031C17.335,6.031,18,6.696,18,7.516L18,7.516z"/>
<path fill="#424242" d="M18,13.516C18,14.335,17.335,15,16.516,15H1.484C0.665,15,0,14.335,0,13.516l0,0 c0-0.82,0.665-1.484,1.484-1.484h15.031C17.335,12.031,18,12.696,18,13.516L18,13.516z"/>
</svg>
</a>
<ul class="nav-list">
<li>
<a class="navhome" href="./">
Strobe-enhanced microscopy stage
</a>
</li>
<li class="active">
<a class="active" href="./3-level-station.html">
Build the 3-level microscopy stage
</a>
<ul class="sub-nav-list">
<li>
<a class="not-active" href="./printing.html">
Print the plastic parts
</a>
</li>
<li>
<a class="active" href="./laser-cutting.html">
Laser cut the acrylic parts
</a>
</li>
<li>
<a class="not-active" href="./assemble-focus-mechanism.html">
Assemble the focus mechanism
</a>
</li>
<li>
<a class="not-active" href="./basic-optics-module.html">
Assemble the basics optics module
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-top-plate.html">
Attach parts to the top plate
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-middle-plate.html">
Attach parts to the middle plate
</a>
</li>
<li>
<a class="not-active" href="./attach-parts-to-bottom-plate.html">
Attach parts to the bottom plate
</a>
</li>
<li>
<a class="not-active" href="./mount-optics.html">
Mount the optics
</a>
</li>
<li>
<a class="not-active" href="./wiring.html">
Mount the electronics
</a>
</li>
<li>
<a class="not-active" href="./illumination.html">
Assemble the illumination
</a>
</li>
<li>
<a class="not-active" href="./finished.html">
Assembly complete
</a>
</li>
</ul>
</li>
<li class="not-active">
<a class="not-active" href="./software/software-installation.html">
Software Installation
</a>
</li>
<li class="not-active">
<a class="not-active" href="./usage.html">
Use your Stage
</a>
</li>
<li class="not-active">
<a class="not-active" href="./troubleshooting.html">
Troubleshooting
</a>
</li>
<li class="not-active">
<a class="not-active" href="./variants_accesories.html">
Customisation and accessories
</a>
</li>
</ul>
</nav>
</div>
<div class="wrapper">
<h1 id="laser-cut-the-acrylic-parts">Laser cut the acrylic parts</h1>
<div class="pagebom">
<h3 id="tool">Tools</h3>
<ul>
<li>1 <a Class="bom" href="parts/tools/laser-cutting-machine.html">Laser cutting machine</a> </li>
</ul>
<h3 id="material">Materials</h3>
<ul>
<li>1 <a Class="bom" href="parts/materials/acrylic-sheet.html">52x18x0.5cm Acrylic sheet</a> </li>
</ul>
</div>
<h2 class="page-step" id="set-your-laser-cutting-machine-settings">Step 1: Set your laser cutting machine settings</h2>
<p>Laser-cutting acrylics is relatively simple. The basic steps for achieving good-quality cut acrylic parts are listed <a href="https://www.xometry.com/resources/sheet/about-laser-cutting-acrylic/">here</a>.</p>
<p>Settings for laser cutting acrylics will vary according to the machine. The table below lists the settings we used: </p>
<table>
<thead>
<tr>
<th>Setting</th>
<th>Value</th>
</tr>
</thead>
<tbody>
<tr>
<td>Material</td>
<td>Acrylic 5mm</td>
</tr>
<tr>
<td>Power (W)</td>
<td>50</td>
</tr>
<tr>
<td>Speed (mm/s)</td>
<td>10</td>
</tr>
</tbody>
</table>
<p>We recommend testing the parameters for acrylic laser cutting using a test matrix. Download and laser cut the <a href="parts/materials/test-matrix.html" title="">test matrix</a> file. This will only use about an 8x9x0.5cm acrylic sheet.</p>
<p>The result should look like this:</p>
<p><img alt="" src="images/test-matrix-1.jpg" title="" /></p>
<h2 class="page-step" id="laser-cutting">Step 2: Laser cutting</h2>
<p>Now you have tested your <a href="parts/tools/laser-cutting-machine.html">laser cutting machine</a> and <a href="parts/materials/acrylic-sheet.html">acrylic sheet</a> you can laser cut the following parts:</p>
<ul>
<li><a name="output__top-plate"></a>Top plate (17x17x0.5cm): <a href="parts/materials/top-plate.html" title="">top-plate.dxf</a></li>
<li><a name="output__middle-plate"></a>Middle plate (17x17x0.5cm): <a href="parts/materials/middle-plate.html" title="">middle-plate.dxf</a></li>
<li><a name="output__bottom-plate"></a>Bottom plate (17x17x0.5cm): <a href="parts/materials/bottom-plate.html" title="">bottom-plate.dxf</a></li>
</ul>
<h3 id="remove-film">Remove film</h3>
<ul>
<li>
<p>If there is protective film on the acrylic, remove it now.</p>
</li>
<li>
<p>Your plates are now prepared and ready to use.</p>
</li>
</ul>
<hr />
<p><a href="printing.html">Previous page</a> | <a href="assemble-focus-mechanism.html">Next page</a></p>
</div>
</div>
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<p class="author">©2024 Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="email">Contact: <a href="mailto:librehub@uc.cl">librehub@uc.cl</a></p>
<p class="license">Strobe-enhanced microscopy stage is released under <a href="license.html">CERN-OHL-S-2.0</a>.</p>
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