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10 changes: 7 additions & 3 deletions _sources/imaging/imaging.rst.txt
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.. _process-home:

###############################
Imaging with the System
Imaging Process
###############################

Visualization of Axes Mapping
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:align: center
:alt: Before and after of adjusting in Z-projections after adjusting the correction collar

Processing Images - Deskewing
###############################
Image Post-Processing
###############################

Deskewing
______________________________


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difference between the normal of our angle mount and the y-axis. If this value is unknown, one can use different
values for the deskew angle until the bead PSFs are correctly aligned along the z-axis and not angled.

Processing Images - Rescaling
Rescaling
______________________________


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3 changes: 2 additions & 1 deletion _templates/custom_class.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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3 changes: 2 additions & 1 deletion _templates/custom_module.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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3 changes: 2 additions & 1 deletion design_principles/design_process.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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3 changes: 2 additions & 1 deletion design_principles/required_software.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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28 changes: 17 additions & 11 deletions imaging/imaging.html
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<meta charset="utf-8" /><meta name="generator" content="Docutils 0.19: https://docutils.sourceforge.io/" />

<meta name="viewport" content="width=device-width, initial-scale=1.0" />
<title>Imaging with the System &mdash; navigate-hardware documentation</title>
<title>Imaging Process &mdash; navigate-hardware documentation</title>
<link rel="stylesheet" type="text/css" href="../_static/pygments.css" />
<link rel="stylesheet" type="text/css" href="../_static/css/theme.css" />

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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul class="current">
<li class="toctree-l1 current"><a class="current reference internal" href="#">Imaging with the System</a><ul>
<li class="toctree-l1 current"><a class="current reference internal" href="#">Imaging Process</a><ul>
<li class="toctree-l2"><a class="reference internal" href="#visualization-of-axes-mapping">Visualization of Axes Mapping</a></li>
<li class="toctree-l2"><a class="reference internal" href="#finding-the-focus">Finding the Focus</a></li>
<li class="toctree-l2"><a class="reference internal" href="#minimizing-spherical-aberrations">Minimizing Spherical Aberrations</a></li>
<li class="toctree-l2"><a class="reference internal" href="#processing-images-deskewing">Processing Images - Deskewing</a></li>
<li class="toctree-l2"><a class="reference internal" href="#processing-images-rescaling">Processing Images - Rescaling</a></li>
</ul>
</li>
<li class="toctree-l1"><a class="reference internal" href="#image-post-processing">Image Post-Processing</a><ul>
<li class="toctree-l2"><a class="reference internal" href="#deskewing">Deskewing</a></li>
<li class="toctree-l2"><a class="reference internal" href="#rescaling">Rescaling</a></li>
</ul>
</li>
</ul>
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<div role="navigation" aria-label="Page navigation">
<ul class="wy-breadcrumbs">
<li><a href="../index.html" class="icon icon-home" aria-label="Home"></a></li>
<li class="breadcrumb-item active">Imaging with the System</li>
<li class="breadcrumb-item active">Imaging Process</li>
<li class="wy-breadcrumbs-aside">
<a href="../_sources/imaging/imaging.rst.txt" rel="nofollow"> View page source</a>
</li>
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<div role="main" class="document" itemscope="itemscope" itemtype="http://schema.org/Article">
<div itemprop="articleBody">

<section id="imaging-with-the-system">
<span id="process-home"></span><h1>Imaging with the System<a class="headerlink" href="#imaging-with-the-system" title="Permalink to this heading"></a></h1>
<section id="imaging-process">
<span id="process-home"></span><h1>Imaging Process<a class="headerlink" href="#imaging-process" title="Permalink to this heading"></a></h1>
<section id="visualization-of-axes-mapping">
<h2>Visualization of Axes Mapping<a class="headerlink" href="#visualization-of-axes-mapping" title="Permalink to this heading"></a></h2>
<p>In our system we essentially have 5 different translation stages at work: the standard x,y, and z axes, an additional
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focused.</p>
<img alt="Before and after of adjusting in Z-projections after adjusting the correction collar" class="align-center" src="../_images/SphericalExample1.png" />
</section>
<section id="processing-images-deskewing">
<h2>Processing Images - Deskewing<a class="headerlink" href="#processing-images-deskewing" title="Permalink to this heading"></a></h2>
</section>
<section id="image-post-processing">
<h1>Image Post-Processing<a class="headerlink" href="#image-post-processing" title="Permalink to this heading"></a></h1>
<section id="deskewing">
<h2>Deskewing<a class="headerlink" href="#deskewing" title="Permalink to this heading"></a></h2>
<p>With an image stack acquired, some post processing is still required in order to remove the effects of shearing in our
images. The root of this shearing is due to the angled method in which our sample is mounted and similarly, the angled path that
the sample moves as the piezo is scanned. A basic visual idea of how deskewing affects the resulting image is shown
Expand All @@ -163,8 +169,8 @@ <h2>Processing Images - Deskewing<a class="headerlink" href="#processing-images-
difference between the normal of our angle mount and the y-axis. If this value is unknown, one can use different
values for the deskew angle until the bead PSFs are correctly aligned along the z-axis and not angled.</p>
</section>
<section id="processing-images-rescaling">
<h2>Processing Images - Rescaling<a class="headerlink" href="#processing-images-rescaling" title="Permalink to this heading"></a></h2>
<section id="rescaling">
<h2>Rescaling<a class="headerlink" href="#rescaling" title="Permalink to this heading"></a></h2>
<p>With a properly deskewed image set, the next step is to work to rescale the image set dimensions to properly
represent the physical pixel sizes in every dimension. The first step to doing this involves going to the
properties tab of the image stack (Image-&gt; Properties) and adjusting each dimension such that the x and y values
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12 changes: 8 additions & 4 deletions index.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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<div class="toctree-wrapper compound">
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html">Imaging with the System</a><ul>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html">Imaging Process</a><ul>
<li class="toctree-l2"><a class="reference internal" href="imaging/imaging.html#visualization-of-axes-mapping">Visualization of Axes Mapping</a></li>
<li class="toctree-l2"><a class="reference internal" href="imaging/imaging.html#finding-the-focus">Finding the Focus</a></li>
<li class="toctree-l2"><a class="reference internal" href="imaging/imaging.html#minimizing-spherical-aberrations">Minimizing Spherical Aberrations</a></li>
<li class="toctree-l2"><a class="reference internal" href="imaging/imaging.html#processing-images-deskewing">Processing Images - Deskewing</a></li>
<li class="toctree-l2"><a class="reference internal" href="imaging/imaging.html#processing-images-rescaling">Processing Images - Rescaling</a></li>
</ul>
</li>
<li class="toctree-l1"><a class="reference internal" href="imaging/imaging.html#image-post-processing">Image Post-Processing</a><ul>
<li class="toctree-l2"><a class="reference internal" href="imaging/imaging.html#deskewing">Deskewing</a></li>
<li class="toctree-l2"><a class="reference internal" href="imaging/imaging.html#rescaling">Rescaling</a></li>
</ul>
</li>
</ul>
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3 changes: 2 additions & 1 deletion introduction/aslm.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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3 changes: 2 additions & 1 deletion introduction/background.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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3 changes: 2 additions & 1 deletion introduction/nanospim.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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3 changes: 2 additions & 1 deletion introduction/opm.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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3 changes: 2 additions & 1 deletion introduction/principles.html
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</ul>
<p class="caption" role="heading"><span class="caption-text">Imaging with the System</span></p>
<ul>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging with the System</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html">Imaging Process</a></li>
<li class="toctree-l1"><a class="reference internal" href="../imaging/imaging.html#image-post-processing">Image Post-Processing</a></li>
</ul>

</div>
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<script src="../_static/js/theme.js"></script>
<link rel="index" title="Index" href="../genindex.html" />
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</div>
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