Code for CD deconvolution into ellipticity
Ellipticity according to:
Greenfield NJ. Using circular dichroism spectra to estimate protein secondary structure. Nat Protoc. 2006;1(6):2876-2890. doi:10.1038/nprot.2006.202
Modify these parameters in the notebook:
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molecular_weight: protein mass in daltons;
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aa: number of amino acids residues;
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path_length: cuvette path in mm;
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molarity: protein concentration in micromolar;
For plotting, there is a google colab notebook attached for CD spectra plotting associated with Savitzky–Golay filter smoothing (optional).
Abraham. Savitzky and M. J. E. Golay Analytical Chemistry 1964 36 (8), 1627-1639 DOI: 10.1021/ac60214a047