Processing circular dichroism data

Deconvolution

Code for CD deconvolution into ellipticity

Ellipticity according to:

Greenfield NJ. Using circular dichroism spectra to estimate protein secondary structure. Nat Protoc. 2006;1(6):2876-2890. doi:10.1038/nprot.2006.202

Usage:

Modify these parameters in the notebook:

• molecular_weight: protein mass in daltons;

• aa: number of amino acids residues;

• path_length: cuvette path in mm;

• molarity: protein concentration in micromolar;

Plotting

For plotting, there is a google colab notebook attached for CD spectra plotting associated with Savitzky–Golay filter smoothing (optional).

Abraham. Savitzky and M. J. E. Golay Analytical Chemistry 1964 36 (8), 1627-1639 DOI: 10.1021/ac60214a047